Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (Lung)
Specification
Lung
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Other product details
Dilution
1/25
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% Blocking serum
Secondary antibody
Name
Non-Abcam antibody was used:
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594 + Gold 1.4nm
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594 + Gold 1.4nm
Dilution
1/200
Additional data
Additional Notes
There is no cross-reactivity with sheep lung tissue and also against human tonsil tissue. The same sections were costained with other another antibody which worked well so there is no problem in the technique.
Abcam response
Thank you for your valuable feedback. This antibody has not been tested on sheep and may not be suitable for use in this species. We welcome other abreviews to determine this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 13 2013