Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cervical tumor cell lines)
Specification
cervical tumor cell lines
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-BSA 5%
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/5000
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投稿 Dec 31 2010