Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS + 0.2% Triton
Secondary antibody
Name
Non-Abcam antibody was used: Donkey-anti-rabbit Alexa 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
Immunostaining obtained with this antibody in the rat spinal cord using the antibody 1:500 diluted. The staining is very good, located around axons as expected.
Immunostaining obtained with this antibody in the rat spinal cord using the antibody 1:500 diluted. The staining is very good, located around axons as expected.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Karine Thibault
Verified customer
投稿 Sep 27 2011