Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Tissue sections (Sciatic nerve)
Specification
Sciatic nerve
Permeabilization
Yes - 0.1% TRITON X-100 in TBS
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
Additional data
Additional Notes
Fresh-frozen sections were cut at 10 microns. After drying for 2hrs in a fumehood, sections were fixed for 10 mins in 10% Formalin.
After rinsing in TBS, sections were permeabilised for 10 mins before incubatibng in primary Ab.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 May 31 2013