Application
Western blot
Sample
Rat Cell lysate - whole cell (prefrontal cortex)
Loading amount
50 µg
Specification
prefrontal cortex
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 18 kDa
Additional data
Abcam response
Thank you for your valuable feedback. According to the Uniprot protein database page for this protein, there are several isoforms. Some of these are at around 18 kDa.
http://www.uniprot.org/uniprot/P02688
http://www.uniprot.org/uniprot/P02688
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 13 2012