Anti-Myc tag 抗体 [9E10] (ab32)
Key features and details
- Mouse monoclonal [9E10] to Myc tag
- Suitable for: ICC/IF, Flow Cyt, WB, IP, ELISA, IHC-Fr, Purification
- Reacts with: Species independent
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-Myc tag antibody [9E10]
Myc tag 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9E10] to Myc tag -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, Flow Cyt, WB, IP, ELISA, IHC-Fr, Purificationmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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エピトープ
Epitope located at aa 410-419; EQKLISEEDL -
ポジティブ・コントロール
- Myc tagged proteins and myc tag expressing cells.
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特記事項
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
9E10 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
- DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
- DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
- DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
- FITC Anti-Myc tag antibody [9E10] (ab117599)
- Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
- Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
- Anti-Myc tag antibody [9E10] (ab206486)
- Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
- Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
- APC Anti-Myc tag antibody [9E10] (ab223896)
- Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
- HRP Anti-Myc tag antibody [9E10] (ab62928)
- PE Anti-Myc tag antibody [9E10] (ab72468)
- SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)
- Biotin Anti-Myc tag antibody [9E10] (ab81658)
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (6) |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (13) |
1/500 - 1/1000.
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IP | (3) |
Use at 6 µg/mg of lysate.
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
1/1000.
See Abreviews. |
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Purification |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/1000. |
IP
Use at 6 µg/mg of lysate. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
1/1000. See Abreviews. |
Purification
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
細胞内局在
Nuclear -
別名
- c-myc tag antibody
- Myc Epitope Tag antibody
画像
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All lanes : Anti-Myc tag antibody [9E10] (ab32) at 1/200 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : MYC CRISPR-Cas9 edited HEK-293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.False colour image of Western blot: Anti-Myc tag antibody [9E10] staining at 1/200 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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Anti-Myc tag antibody [9E10] (ab32) at 1 µg/ml +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteLysate from E. coli recombinantly expressing 11 commonly used tags including myc tag.
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Ab32 staining a Myc tagged protein in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Endogenous c-myc was not detected under these conditions. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton and blocked with 5% Serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in 5% Serum) for 1 hour at 25°C. An Alexa Fluor® 488 conjugated Goat anti-Mouse was used as a secondary antibody.
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Phosphorylation of Cdc15 changes during the cell cycle. Exponentially growing cells (cyc) of CDC15-MYC9 (W1114) were arrested in G1 with a factor pheromone and released into fresh medium at 25°C. Cells were harvested at the indicated times, the percentage of divided nuclei was determined by DAPI staining of fixed cells, and proteins were analyzed by western blotting with 9E10 (ab32).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (403)
ab32 は 403 報の論文で使用されています。
- Xu WN et al. Sesn2 Serves as a Regulator between Mitochondrial Unfolded Protein Response and Mitophagy in Intervertebral Disc Degeneration. Int J Biol Sci 19:571-592 (2023). PubMed: 36632468
- Feng T et al. OsMADS14 and NF-YB1 cooperate in the direct activation of OsAGPL2 and Waxy during starch synthesis in rice endosperm. New Phytol 234:77-92 (2022). PubMed: 35067957
- Sengupta S et al. GABA transmission from mAL interneurons regulates aggression in Drosophila males. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35082150
- Cohen A et al. TOR complex 2 contributes to regulation of gene expression via inhibiting Gcn5 recruitment to subtelomeric and DNA replication stress genes. PLoS Genet 18:e1010061 (2022). PubMed: 35157728
- Li X et al. Regulation of P53 signaling in breast cancer by the E3 ubiquitin ligase RNF187. Cell Death Dis 13:149 (2022). PubMed: 35165289