Anti-MyD88 抗体 (ab28763)
Key features and details
- Goat polyclonal to MyD88
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-MyD88 antibody
MyD88 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MyD88 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, ICC/IF, WB, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Cow, Dog -
免疫原
Synthetic peptide:
C-IKYKAM KKEFP
, corresponding to Internal sequence amino acids 255-265 of Human MyD88. -
ポジティブ・コントロール
- IHC-P: Human tonsil; ICC/IF: Jurkat and U2OS cells; Flow Cyt: Jurkat cells; WB: Human spleen lysate;
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28763の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
1 hour primary incubation is recommended for this product. |
|
Flow Cyt |
Use a concentration of 10 µg/ml.
|
特記事項 |
---|
IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). 1 hour primary incubation is recommended for this product. |
Flow Cyt
Use a concentration of 10 µg/ml. |
ターゲット情報
-
機能
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. -
組織特異性
Ubiquitous. -
関連疾患
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. -
配列類似性
Contains 1 death domain.
Contains 1 TIR domain. -
ドメイン
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 444881 Cow
- Entrez Gene: 4615 Human
- Entrez Gene: 17874 Mouse
- Entrez Gene: 301059 Rat
- Entrez Gene: 678682 Sheep
- Omim: 602170 Human
- SwissProt: Q599T9 Cow
- SwissProt: Q99836 Human
see all -
別名
- Mutant myeloid differentiation primary response 88 antibody
- MYD 88 antibody
- Myd88 antibody
see all
画像
-
Anti-MyD88 antibody (ab28763) at 0.5 µg/ml + Human Spleen lysate at 35 µg
Predicted band size: 33 kDaLysates in RIPA buffer. Detected by chemiluminescence.
-
ab28763 (4µg/ml) staining of paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
-
-
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
-
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (7)
ab28763 は 7 報の論文で使用されています。
- Chang ZY et al. Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35269806
- Luo Q et al. Long Noncoding RNA MEG3-205/Let-7a/MyD88 Axis Promotes Renal Inflammation and Fibrosis in Diabetic Nephropathy. Kidney Dis (Basel) 8:231-245 (2022). PubMed: 35702702
- Zhou X et al. Intestinal accumulation of microbiota-produced succinate caused by loss of microRNAs leads to diarrhea in weanling piglets. Gut Microbes 14:2091369 (2022). PubMed: 35758253
- Ren AQ et al. Glycoproteins From Rabdosia japonica var. glaucocalyx Regulate Macrophage Polarization and Alleviate Lipopolysaccharide-Induced Acute Lung Injury in Mice via TLR4/NF-κB Pathway. Front Pharmacol 12:693298 (2021). PubMed: 34366849
- Yang C et al. Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway. Stem Cells Int 2020:2134565 (2020). PubMed: 32300366
- Liu D et al. MyD88 expression in the rat dental follicle: implications for osteoclastogenesis and tooth eruption. Eur J Oral Sci 118:333-41 (2010). PubMed: 20662905
- Szajnik M et al. TLR4 signaling induced by lipopolysaccharide or paclitaxel regulates tumor survival and chemoresistance in ovarian cancer. Oncogene 28:4353-63 (2009). IHC-P ; Human . PubMed: 19826413