Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)
Key features and details
- Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Mouse
- Isotype: IgG2b
- Suitable for: ELISA, WB
Related conjugates and formulations
製品の概要
-
製品名
Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)
IgG Light chain 二次抗体 製品一覧 -
由来種
Mouse -
ターゲット生物種
Rabbit -
特異性
ab99696 reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50 kD). -
アプリケーション
適用あり: ELISA, WBmore details -
免疫原
Full length protein corresponding to Rabbit IgG Light chain. Rabbit Light Chain
-
標識
Alkaline Phosphatase
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 8.00
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.0095% Magnesium chloride, 0.605% Tris -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SB62a -
アイソタイプ
IgG2b -
軽鎖の種類
lambda -
研究分野
関連製品
-
Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99696の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
1/2000 - 1/4000.
|
|
WB |
Use at an assay dependent concentration.
|
特記事項 |
---|
ELISA
1/2000 - 1/4000. |
WB
Use at an assay dependent concentration. |
画像
-
Western blot showing that Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) specifically binds to light-chains
Left blot: Coomassie stain showing the rabbit heavy (~50kDa) and light-chains (~25 kDa)
Lane 1: 10 µg rabbit monoclonal was loaded
Lane 2: 5 µg rabbit monoclonal was loaded
Lane 3: 2 µg rabbit monoclonal was loadedRight blot: WB using Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) as secondary antibody at 1/10,000
Lane 1: 1 µg rabbit monoclonal was loaded
Lane 2: 0.5 µg rabbit monoclonal was loaded
Lane 3: 0.25 µg rabbit monoclonal was loaded -
All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/5000 dilution
Lanes 3 - 4 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/20000 dilution
Lanes 5 - 6 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab99696 は 2 報の論文で使用されています。
- Ao X et al. lincRNA-p21 inhibits the progression of non-small cell lung cancer via targeting miR-17-5p. Oncol Rep 41:789-800 (2019). PubMed: 30535441
- Castro-Severyn J et al. Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study. Front Microbiol 10:2161 (2019). PubMed: 31611848