Mouse and Rabbit Specific HRP/DAB Detection IHC kit (ab64264)
製品の概要
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製品名
Mouse and Rabbit Specific HRP/DAB Detection IHC kit
HRP/DAB detection kit キット 製品一覧 -
製品の概要
Mouse and Rabbit specific HRP/DAB Detection IHC Kit ab64264 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB. -
特記事項
For alternative streptavidin-biotin IHC detection kits see:
- Anti-Rabbit HRP-DAB IHC kit ab64261
- Anti-Mouse HRP-DAB IHC Kit ab64259For high sensitivity HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
- Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469Alternatively, see HRP-AEC IHC detection kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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アプリケーション
適用あり: IHC-Pmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 15 ml 50x DAB Chromogen 1 x 0.5ml Biotinylated Goat Anti-Polyvalent 1 x 15ml DAB substrate 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
研究分野
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関連性
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64264の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent dilution.
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特記事項 |
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IHC-P
Use at an assay dependent dilution. |
画像
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Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution and visualized using mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (174)
ab64264 は 174 報の論文で使用されています。
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- Bagatella S et al. Bovine neutrophil chemotaxis to Listeria monocytogenes in neurolisteriosis depends on microglia-released rather than bacterial factors. J Neuroinflammation 19:304 (2022). PubMed: 36527076
- Mito T et al. Mosaic dysfunction of mitophagy in mitochondrial muscle disease. Cell Metab 34:197-208.e5 (2022). PubMed: 35030325