Mouse Osteopontin ELISA Kit (ab100734)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Osteopontin ELISA Kit
Osteopontin キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 4 pg/ml -
検出範囲
2.74 pg/ml - 2000 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 85.46 78% - 105% Serum 115.6 103% - 122% Plasma 110.4 87% - 130% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s Osteopontin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Osteopontin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Mouse Osteopontin 2 vials Osteopontin Microplate (12 x 8 wells) 1 unit Recombinant Mouse Osteopontin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
組織特異性
Bone. Found in plasma. -
配列類似性
Belongs to the osteopontin family. -
翻訳後修飾
Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted. - Information by UniProt
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別名
- BNSP
- Bone sialoprotein 1
- Bone sialoprotein I
see all -
参照データベース
- Entrez Gene: 20750 Mouse
- SwissProt: P10923 Mouse
- Unigene: 288474 Mouse
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab100734 は 5 報の論文で使用されています。
- Mukherjee P et al. Oxidized phospholipids cause changes in jejunum mucus that induce dysbiosis and systemic inflammation. J Lipid Res 63:100153 (2022). PubMed: 34808192
- Dai B et al. Macrophages in epididymal adipose tissue secrete osteopontin to regulate bone homeostasis. Nat Commun 13:427 (2022). PubMed: 35058428
- Trotter TN et al. Adipocyte-Lineage Cells Support Growth and Dissemination of Multiple Myeloma in Bone. Am J Pathol 186:3054-3063 (2016). PubMed: 27648615
- Wong TM et al. Respiratory syncytial virus (RSV) infection in elderly mice results in altered antiviral gene expression and enhanced pathology. PLoS One 9:e88764 (2014). PubMed: 24558422
- Fang X et al. Ameliorated ConA-induced hepatitis in the absence of PKC-theta. PLoS One 7:e31174 (2012). Mouse . PubMed: 22347449