Mouse Mesenchymal Stromal Cell Marker Panel (CD44, CD90, Sca-1, CD45 and CD29) (ab93759)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse Mesenchymal Stromal Cell Marker Panel (CD44, CD90, Sca-1, CD45 and CD29) (ab93759)

IHC image of ab25340 staining CD44 on Mouse Tissue sections (T-cell lymphoma). The cells were 4% PFA fixed (10 min) and then incubated in Perkin-Elmer TNB Blocking Reagent at 0.5% as blocking agent for 30 mins at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25340, 1/250 dilution) overnight at +4°C. The secondary antibody was ab7097.

This image was submitted as part of an anonymous Abreview

  Immunocytochemistry/ Immunofluorescence - Mouse Mesenchymal Stromal Cell Marker Panel (CD44, CD90, Sca-1, CD45 and CD29) (ab93759)

ICC/IF image of ab52971 staining CD29 in Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52971, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody can be used as a marker of CD29 positive human or mouse Mesenchymal Stromal Cells.

  Immunocytochemistry/ Immunofluorescence - Mouse Mesenchymal Stromal Cell Marker Panel (CD44, CD90, Sca-1, CD45 and CD29) (ab93759)

ICC/IF image of ab51317 staining Sca-1 in mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).