You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC)
IgG 抗体 (1691件) 一覧

Target species

Mouse

Tested applications

IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details

Immunogen

mouse whole molecule

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Conjugation notes

Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG

Clonality

Polyclonal

  • Flow Cytometry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)Flow Cytometry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785) image (enlarge)

  • Immunocytochemistry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)Immunocytochemistry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC (PFA fixed)

     IHC (PFA fixed): 1/1000

    IHC (PFA fixed): 1/1000

  • ShowHide

    Immunomicroscopy

     IM: Use at an assay depe...Read more →

    IM: Use at an assay dependent concentration.

  • ShowHide1 Image

    Flow Cyt

     Flow Cyt: 1/500 - 1/2500...Read more →

    Flow Cyt: 1/500 - 1/2500.(PubMed: 17989178)

  • ShowHide1 Image

    IHC-P

     IHC-P: Use at an assay d...Read more →

    IHC-P: Use at an assay dependent concentration.

  • ShowHide

    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent concentration. (PubMed: 15716365For PFA fixed tissue see PMID 15716365.)

  • ShowHide

    ICC/IF

     ICC/IF: 1/1000 - 1/5000....Read more →

    ICC/IF: 1/1000 - 1/5000.((PMID16006199))

  • ShowHide

    ELISA

     ELISA: 1/10000 - 1/50000.Read more →

    ELISA: 1/10000 - 1/50000.

  • ShowHide

    WB

     WB: 1/10000(PubMed: 1721...Read more →

    WB: 1/10000(PubMed: 17217624)

Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) images:

  Flow Cytometry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)

Flow Cytometry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)

Ab6785 was used at dilution 1/100 with the primary antibody ab52455 in Flow Cyt. See the review on ab52455.

  Immunocytochemistry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)

Immunocytochemistry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)

Ab6785 was used at dilution 1/2000 with the primary antibody ab1220 in ICC/IF. See the review on ab1220.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)

Ab6785 was used at dilution 1/300 with the primary antibody ab6002 in IHC-P. See the review on ab6002.

References for Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)

This product has been referenced in:

  • Kästle Met al. Combination of PDT and inhibitor treatment affects melanoma cells and spares keratinocytes. Free Radic Biol Med 50:305-12 (2011). Flow Cyt.Read more (PubMed: 21078385) »
  • Gonzalez-Granillo Met al. Studies of the role of tubulin beta II isotype in regulation of mitochondrial respiration in intracellular energetic units in cardiac cells. J Mol Cell Cardiol : (2011).Read more (PubMed: 21846472) »

See all 19 publications for this product

Publishing research using ab6785? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"