Mouse IgG secondary antibody - H&L (ab6785)

Product Name 

Mouse IgG secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Goat polyclonal to Mouse IgG - H&L (FITC)

Immunogen 

mouse whole molecule

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, IM, WB

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent concentration (PMID 17989178).
ICC/IF: Suggested working dilution of 1/4000 (see PMID: 16006199).
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IHC (PFA fixed): 1/1000 (from PubMed:15716365).
IM: Use at an assay dependent concentration.
WB: 1/10000 (from PubMed: 17217624).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Please refer to the relevant conjugation for dilutions specific for this product:

Research areas 

Secondary antibodies >> anti-Mouse >> IgG >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Raised in 

Goat

Clonality 

Polyclonal

Purity 

IgG fraction

Conjugation notes 

Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons)
Absorption Wavelength: 495 nm
Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Mouse IgG secondary antibody - H&L (ab6785)

  This product has been used in: (two most recent references)

Farrand AJ et al. Only two amino acids are essential for cytolytic toxin recognition of cholesterol at the membrane surface. Proc Natl Acad Sci U S A 107:4341-6 (2010). Flow Cyt. PubMed: 20145114

Jiang S et al. Differential expression of stem cell markers in human follicular bulge and interfollicular epidermal compartments. Histochem Cell Biol 133:455-65 (2010). IHC-Fr. PubMed: 20229054

See all 16 publication references for this product.


If you publish research using ab6785 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Mouse IgG secondary antibody - H&L

Customer FAQs regarding Mouse IgG secondary antibody - H&L

Protocols for Mouse IgG secondary antibody - H&L

Price and availability of products related to Mouse IgG secondary antibody - H&L