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Product Name
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Mouse IgG secondary antibody - H&L
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Product type
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Secondary antibodies
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Description
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Goat polyclonal to Mouse IgG - H&L (FITC)
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Immunogen
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mouse whole molecule
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, IM, WB
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Application notes
(see key)
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Recommended dilutions Flow Cyt: Use at an assay dependent concentration (PMID 17989178). ICC/IF: Suggested working dilution of 1/4000 (see PMID: 16006199). IHC-P: Use at an assay dependent concentration. IHC-Fr: Use at an assay dependent concentration. IHC (PFA fixed): 1/1000 (from PubMed:15716365). IM: Use at an assay dependent concentration. WB: 1/10000 (from PubMed: 17217624).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Mouse >> IgG >> Fluorophore >> FITC Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Relevance
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Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
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Mouse IgG secondary antibody - H&L (ab6785)の別名
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Raised in
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Goat
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG
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Storage buffer
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Preservative: 0.01% Sodium Azide Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Form
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Liquid
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Conjugation
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Fluorescein
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 Mouse IgG secondary antibody - H&L について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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References for Mouse IgG secondary antibody - H&L (ab6785)
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This product has been used in: (two most recent references)
Farrand AJ et al. Only two amino acids are essential for cytolytic toxin recognition of cholesterol at the membrane surface. Proc Natl Acad Sci U S A 107:4341-6 (2010). Flow Cyt. PubMed: 20145114
Jiang S et al. Differential expression of stem cell markers in human follicular bulge and interfollicular epidermal compartments. Histochem Cell Biol 133:455-65 (2010). IHC-Fr. PubMed: 20229054
See all 16 publication references for this product.
If you publish research using ab6785 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgG
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