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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC)
IgG 抗体 (1691件) 一覧
Target species
Mouse
Tested applications
IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details
Immunogen
mouse whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Mouse >> IgG >> Fluorophore >> FITC
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Flow Cytometry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
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Immunocytochemistry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab6785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed)
IHC (PFA fixed): 1/1000IHC (PFA fixed): 1/1000
IM: Use at an assay dependent concentration.
Flow Cyt: 1/500 - 1/2500.(PubMed: 17989178)
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration. (PubMed: 15716365For PFA fixed tissue see PMID 15716365.)
ICC/IF: 1/1000 - 1/5000.((PMID16006199))
ELISA: 1/10000 - 1/50000.
WB: 1/10000(PubMed: 17217624)
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) images:
Flow Cytometry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
Ab6785 was used at dilution 1/100 with the primary antibody ab52455 in Flow Cyt. See the review on ab52455.
Immunocytochemistry - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
Ab6785 was used at dilution 1/2000 with the primary antibody ab1220 in ICC/IF. See the review on ab1220.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
Ab6785 was used at dilution 1/300 with the primary antibody ab6002 in IHC-P. See the review on ab6002.
References for Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)
This product has been referenced in:
- Kästle Met al. Combination of PDT and inhibitor treatment affects melanoma cells and spares keratinocytes. Free Radic Biol Med 50:305-12 (2011). Flow Cyt.Read more (PubMed: 21078385) »
- Gonzalez-Granillo Met al. Studies of the role of tubulin beta II isotype in regulation of mitochondrial respiration in intracellular energetic units in cardiac cells. J Mol Cell Cardiol : (2011).Read more (PubMed: 21846472) »
See all 19 publications for this product
Publishing research using ab6785? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"