Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785)

We’ve packaged the other antibody up and are awaiting FedEx pickup.

Thanks for sending the correct antibody asap.

ANSWER:

Thank you for your reply and for returning the vial of ab6785.

The vial of ab6717 will be arriving tomorrow on the order ***.


Please let me know if you need anything else, and have a wonderful evening.

Thanks. It is the autofluorescence , causing the problem. I have looked
at the section without antibodies & it fluoresces. I am reading the
articles, hope will come over the problem.

ANSWER:

Thank you for the follow-up. The article may help, but I think an easy work-around is to try the red channel instead of green, if that is an option. Autofluorescence is generally less of an issue in the red, but you will need a red secondary, such as ab96881 (equivalent to Texas Red or Alexa Fluor 594):

Click here (or use the following: http://www.abcam.com/index.html?datasheet=96881).

You will want to check that unstained section to see if the background autofluorescence is a problem in that channel though.

Interested in using ab63983 on frozen mouse brain tissue sections.

ANSWER:

Thank you for your call today and for your questions. Please see below for information about our testing discount program, and let me know if you have any further questions about these antibodies or our IHC protocols.

Sinceab63983 has not been tested frozen tissue sections (IHC-Fr), I can offer a discount off a future purchase if you buy ab63983 now, test it inIHC-Fr and submit feedback to us in the form of an Abreview. The discount would be worth 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab63983 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab63983 so please wait for my reply before ordering.

2. Purchase ab63983 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-Fr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab63983 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Can Goat polyclonal Secondary Antibody to Mouse IgA - alpha chain (FITC) (ab97234) be used as seondary antibody for "Anti-Francisella tularensis LPS antibody [FB11] (ab2033) in indirect immunofluorescence assay?

ANSWER:

Thank you for your inquiry.


We would not recommend ab97234 as a secondary for ab2033 because they do not match in isotype. The secondary ab97234 targets Mouse IgA, but the primary ab2033 is IgG2a.


I would reccomend using ab6785 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) instead.


http://www.abcam.com/Mouse-IgG-secondary-antibody-H-L-ab6785.html


I hope this information helps. Please contact us with any other questions.

I want to do an IF application on mouse tissue using a mouse anti-Helicobacter pylori antibody as primary antibody and a goat polyclonal secondary antibody to mouse IgG (FITC conjugated). Do you think it should work? I would like to thank you in advance With kind regards

ANSWER:

Thank you for contacting Abcam. I believe that the staining that you wish to do would work, however by using a mouse primary antibody on mouse tissue, you do run the risk of having high background. I have attached the Abcam protocol for mouse on mouse staining, which will give you information on how to prevent the high background. One popular method is to directly conjugate your primary antibody to a fluorescent tag. We do sell a number of these kits, please see the link below: http://www.abcam.com/easylink If there is anything else I can help you with, please let me know.