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Product Name
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Mouse IgG secondary antibody - H&L
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Product type
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Secondary antibodies
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Description
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Rabbit polyclonal to Mouse IgG - H&L (HRP)
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Immunogen
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Mouse IgG whole molecule
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Tested applications
(see key)
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Dot, ELISA, IHC-Fr, IHC-P, IM, WB
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Application notes
(see key)
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Recommended dilutions
WB: 1/2000 (from PubMed:17200442).
suitable for immunoblotting (dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Mouse >> IgG >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Relevance
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Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
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Mouse IgG secondary antibody - H&L (ab6728)の別名 |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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Horseradish Peroxidase (HRP)
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Storage buffer
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Preservative: 0.01% Gentamicin Sulfate
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 Mouse IgG secondary antibody - H&L について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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References for Mouse IgG secondary antibody - H&L (ab6728)
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This product has been used in: (two most recent references)
Tsuhako MH et al. Tempol ameliorates murine viral encephalomyelitis by preserving the blood-brain barrier, reducing viral load, and lessening inflammation. Free Radic Biol Med 48:704-12 (2010). Dot. PubMed: 20035861
Pizzuto R et al. Pyruvate kinase in pig liver mitochondria. Arch Biochem Biophys 495:42-8 (2010). WB. PubMed: 20026031
See all 16 publication references for this product.
If you publish research using ab6728 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgG
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