|
|
|
|
|
Product Name
|
Mouse IgG secondary antibody - H&L
|
|
Product type
|
Secondary antibodies
|
|
Description
|
Rabbit polyclonal to Mouse IgG - H&L (TR)
|
|
Immunogen
|
Mouse IgG whole molecule
|
|
Tested applications
(see key)
|
Flow Cyt, IHC-Fr, IHC-P, IM
|
|
Application notes
(see key)
|
Recommended dilutions
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
|
|
|
Research areas
|
Secondary antibodies >> anti-Mouse >> IgG >> Fluorophore >> Texas Red
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
|
|
Relevance
|
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
|
|
|
Mouse IgG secondary antibody - H&L (ab6726)の別名 |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Purity
|
IgG fraction
|
|
Conjugation notes
|
Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons)
Absorption Wavelength: 596 nm
Emission Wavelength: 620 nm
Fluorochrome/Protein Ratio: 2.1 moles Texas Redä per mole of Rabbit IgG
|
|
Storage buffer
|
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
|
|
Form
|
Liquid
|
|
Conjugation
|
Texas Red ®
|
|
Concentration
|
2.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Notes
|
Texas RedTM is a registered trademark of Molecular Probes Inc.
|
| |
Abcam では全ての製品情報を一元管理しており、 Mouse IgG secondary antibody - H&L について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
 |
|
References for Mouse IgG secondary antibody - H&L (ab6726)
|
|
This product has been used in:
Pfender NA et al. Route of Uptake of Palmitoylated Encephalitogenic Peptides of Myelin Proteolipid Protein by Antigen-Presenting Cells: Importance of the Type of Bond between Lipid Chain and Peptide and Relevance to Autoimmunity. J Immunol 180:1398-404 (2008). ICC/IF. PubMed: 18209034
If you publish research using ab6726 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to IgG
|
| |
Mouse IgG secondary antibody - H&L - more information
|
Customer reviews (feedback) regarding Mouse IgG secondary antibody - H&L |
Customer FAQs regarding Mouse IgG secondary antibody - H&L |
Protocols for Mouse IgG secondary antibody - H&L |
Price and availability of products related to Mouse IgG secondary antibody - H&L |
| |
Mouse IgG secondary antibody - H&L - other tools:
|
Contact Abcam with a Technical Enquiry about Mouse IgG secondary antibody - H&L |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
