Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy5 ®), pre-adsorbed (ab6563)

Not all of them have that info listed. So I am just wondering whether antibody-binding proteins (Protein A, G and L) were used for purification.

ANSWER:

Thank you for contacting Abcam.

All of the information regarding purification of the antibodies in our catalogue is available on our website. If we do not specify that Protein A/G was used in the purification process then they were not used to purify the antibody.

I have looked through the list you sent and for some there is no purification notes, as is the case for ab3354 as it is supplied as an Ascites fluid but for ab3356, it does say that Protein G was used for purification purposes.

Please let me know if there is anything else I can help you with.

Thank you, John.

Would you also tell me whether antibodies listed below were purified all by antibody-binding proteins (protein A or G) immobilized on beads?






ab3354



ab20137



ab3359



ab3967



ab3356



ab3982



ab3968



ab3357



ab24005



ab76754

ANSWER:

Thank you for your reply.

The information for how each antibody is purified is available on our website. Just go to the webpage for each antibody and the information is there. I have attached a document that shows you where to find that information.

If you have anymore questions after looking at the webpages, please let me know.

Hi,
Would you tell me how this antibody was purified?
Thanks!

ANSWER:

Thank you for contacting Abcam.

The antibody ab6563 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.

Please let me know if there is anything else I can help you with.

Hi, May I know the difference between ab97037 & ab6563? Can either one be used for western blots? Thank you for your help.

ANSWER:

Thank you very much for your interest in our antibodies.

The difference between these two secondary antibodies it that ab6563 is pre-adsorbed against 10 species (chicken, cow, goat, guinea pig, hamster, horse, human, rabbit, rat, sheep), while ab97037 is pre-adsorbed against only 8 species (chicken, cow, goat, horse, human, rabbit, rat, sheep). It will depend on your lysate, which one would be better suited. If you are using a human cell line for example ab6563 would be better suited.

Although we have not tested these antibodies, we believe they would work and could be used in Western blot, provided you have the appropriate equipment for the detection of Cy dyes. Please do not hesitate to contact us or the manufacturer of your detection system for more information.

Given this, I can offer a discount off a future purchase if you buy ab6563 or ab97037 now, test it in Western blot and submit feedback to us. It doesn’t matter whether the feedback is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free secondary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab6563 or ab97037 in Western blot. I will then send a discount code. This code must be issued before purchasing ab so please wait for my reply before ordering.

2. Purchase ab6563 or ab97037 either by phone, fax, or online (www.abcam.com).

3. Test it in Western blot.

4. Let us know the results, positive or negative, preferably via an email to technical@abcam.com (images are great if you have them!) similar to our Abreview system (which can be found here: http://www.abcam.com/abreviews.)

5. After the feedback is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any secondary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for Western blot, you will still receive the discount on your next purchase after your feedback has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

Otherwise, if you are not interested sharing your results, i.e. in the above mentioned testing discount, please let me know and I could arrange for a discount when you place the order.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Blue precipitate has formed after being stored at 4C for a couple weeks.

ANSWER:

Thank you for your phone call yesterday and for your questions about ab6563.

I have been in touch with the lab, and I was told that small amounts of precipitate are common and should not affect the viability of the antibody. It is possible that the antibody was damaged if it went through a few freeze-thaw cycles, but it should be fine stored at 4C for couple weeks.

If you