Mouse IL-28 ELISA Kit (ab100708)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2.46 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IL-28 ELISA Kit -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
2.46 pg/ml - 600 pg/ml -
添加回収試験
88 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 74.3 67% - 87% Serum 109.8 98% - 118% Plasma 76.8 67% - 93% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IL-28 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-28 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for IL-28 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-28 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Mouse IL-28 2 vials IL-28 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-28 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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別名
- Cytokine Zcyto20
- IFN lambda 2
- IFNL2
see all -
参照データベース
- Entrez Gene: 338374. Mouse
- SwissProt: Q4VK74 Mouse
- SwissProt: Q8CGK6 Mouse
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab100708 は 1 報の論文で使用されています。
- Okamoto M et al. IPS-1 is essential for type III IFN production by hepatocytes and dendritic cells in response to hepatitis C virus infection. J Immunol 192:2770-7 (2014). PubMed: 24532585