Mouse CXCL5 ELISA Kit (GCP-2) (ab100719)
Key features and details
- Sensitivity: 35 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse CXCL5 ELISA Kit (GCP-2)
CXCL5 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 35 pg/ml -
検出範囲
31.25 pg/ml - 2000 pg/ml -
添加回収試験
91 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 83.94 74% - 94% Serum 98.05 86% - 106% Plasma 93.11 83% - 102% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s CXCL5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CXCL5 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for CXCL5 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Mouse CXCL5 2 vials CXCL5 Microplate (12 x 8 wells) 1 unit Recombinant Mouse CXCL5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性
CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation. -
別名
- AMCF II
- AMCFII
- C X C motif chemokine 5
see all -
参照データベース
- SwissProt: P50228 Mouse
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab100719 は 4 報の論文で使用されています。
- Simoncello F et al. CXCL5-mediated accumulation of mature neutrophils in lung cancer tissues impairs the differentiation program of anticancer CD8 T cells and limits the efficacy of checkpoint inhibitors. Oncoimmunology 11:2059876 (2022). PubMed: 35402081
- Xu M et al. Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation. Mol Metab 34:112-123 (2020). PubMed: 32180551
- Rao S et al. A dual role for autophagy in a murine model of lung cancer. Nat Commun 5:3056 (2014). PubMed: 24445999
- Lee CH et al. Glutamine suppresses airway neutrophilia by blocking cytosolic phospholipase A(2) via an induction of MAPK phosphatase-1. J Immunol 189:5139-46 (2012). Mouse . PubMed: 23109722