Mouse CXCL16 ELISA Kit (ab100677)
Key features and details
- Sensitivity: 1.3 pg/ml
- Range: 1.25 pg/ml - 80 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
-
製品名
Mouse CXCL16 ELISA Kit
CXCL16 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall < 12% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 1.3 pg/ml -
検出範囲
1.25 pg/ml - 80 pg/ml -
添加回収試験
94 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 96.71 84% - 103% Serum 94.34 83% - 103% Plasma 93.49 82% - 102% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s CXCL16 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL16 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CXCL16 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL16 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CXCL16 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL16 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CXCL16 2 vials CXCL16 Microplate (12 x 8 wells) 1 unit Recombinant Mouse CXCL16 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
関連性
CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. It induces a strong chemotactic response and calcium mobilization. It binds to CXCR6/Bonzo. -
細胞内局在
Cell Membrane and Secreted -
別名
- 0910001K24Rik
- AV290116
- BB024863
see all -
参照データベース
- Entrez Gene: 66102 Mouse
- SwissProt: Q8BSU2 Mouse
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab100677 は 2 報の論文で使用されています。
- Kuriyama H et al. Immunotherapy with 4-1BBL-Expressing iPS Cell-Derived Myeloid Lines Amplifies Antigen-Specific T Cell Infiltration in Advanced Melanoma. Int J Mol Sci 22:N/A (2021). PubMed: 33669419
- Wu J et al. Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke. Aging Dis 12:786-800 (2021). PubMed: 34094642