Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (H1299 Lung cancer cells)
Specification
H1299 Lung cancer cells
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: CF568 Donkey Anti-rabbit IgG(H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Dimitra Kalamida
Verified customer
投稿 Feb 27 2015