Application
Western blot
Sample
Mouse Cell lysate - whole cell (glioma cell, placenta, MDCK, HEK, HL-1)
Loading amount
35 µg
Specification
glioma cell, placenta, MDCK, HEK, HL-1
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
FCS as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
3 hour(s) and 0 minute(s) · Diluent: TBS/Tween
Secondary antibody
Name
Non-Abcam antibody was used: goat-anti rabbit-HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 40 kDa Non-specific: 48, 5 kDa
Positive control
human placenta, human glioma cell line
Additional data
Additional Notes
100%ABR-T7BPU
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Nicole Basler
Verified customer
投稿 Aug 04 2011