Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (Bis-Tris 12%)
Sample
Cynomolgus Monkey Tissue lysate - whole (Peripheral Blood Mononuclear Cell)
Specification
Peripheral Blood Mononuclear Cell
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat-anti-mouse
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
Pierce Supersignal West Pico
Bands
Specific: 80 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
Monkey PBMC isolated from blood
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Daniel Tyrrell
Verified customer
投稿 Nov 22 2013