Application
Western blot
Sample
Mouse Cell lysate - other (Isolated brain mitochondria)
Loading amount
20 µg
Specification
Isolated brain mitochondria
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat milk
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IGg HRP Conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
TMB
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~85 kDa
Positive control
Isolated mitochondria from whole brain
Additional data
Additional Notes
I didn't get any bands with ECL though.
But TMB worked really great.
But TMB worked really great.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Jia Yao
Verified customer
投稿 Sep 17 2009