Anti-Mitofusin 2 + Mitofusin 1 抗体 [3C9] (ab57602)
Key features and details
- Mouse monoclonal [3C9] to Mitofusin 2 + Mitofusin 1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human, Cynomolgus monkey
- Isotype: IgG2a
製品の概要
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製品名
Anti-Mitofusin 2 + Mitofusin 1 antibody [3C9] -
製品の詳細
Mouse monoclonal [3C9] to Mitofusin 2 + Mitofusin 1 -
由来種
Mouse -
特異性
The immunogen used for this product shares 63% homology with MFN2.
ab57602 binds to Mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2) in western blot.
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アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details -
種交差性
交差種: Mouse, Rat, Human, Cynomolgus monkey -
免疫原
Recombinant full length protein corresponding to Human Mitofusin 1 aa 1-741.
Database link: Q8IWA4 -
ポジティブ・コントロール
- WB: Mouse cardiomyocytes whole cell lysate; Hela whole cell extract. ICC/IF: HepG2 cells IHC-P: Human normal kidney. Flow cyt: HEK293 cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 05 Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituents: 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Monobasic dihydrogen potassium phosphate, 0.2% Potassium chloride, 91% Water -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
3C9 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab57602の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (7) |
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
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IHC-P | (4) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (3) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IP |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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細胞内局在
Mitofusin 2: Mitochondrion outer membrane. Colocalizes with BAX during apoptosis. Mitofusin 1: Cytoplasm and Mitochondrion outer membrane. -
参照データベース
- Entrez Gene: 55669 Human
- Entrez Gene: 9927 Human
- Entrez Gene: 170731 Mouse
- Entrez Gene: 67414 Mouse
- Entrez Gene: 192647 Rat
- Entrez Gene: 64476 Rat
- Omim: 608506 Human
- Omim: 608507 Human
see all
画像
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All lanes : Anti-Mitofusin 2 + Mitofusin 1 antibody [3C9] (ab57602) at 1/1000 dilution
Lane 1 :Recombinant Human Mitofusin 1 protein (ab132635)
Lane 2 : MFN2 OE lysate (DDK tag) 10ng
Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution
Predicted band size: 84 kDaThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab57602 overnight at 4°C. Antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
The difference in MW is due to lane one having a GST tag and lane 2 a DDK tag.
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ICC/IF image of ab57602 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57602, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using a version of the antibody produced in ascites.
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All lanes : Anti-Mitofusin 2 + Mitofusin 1 antibody [3C9] (ab57602) at 1/1000 dilution
All lanes : Mouse cardiomyocytes whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-mouse IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocked with 5% milk for 1 hour at 25°C.
Incubated with the primary antibody at 4°C for 13 hours in 1X TBS.
This image was generated using a version of the antibody produced in ascites.
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Mitofusin 2 + Mitofusin 1 antibody (ab57602) at 1ug/lane + HeLa cell lysate at 25 μg/lane.
This image was generated using a version of the antibody produced in ascites.
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IHC image of ab57602 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab57602, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.This image was generated using a version of the antibody produced in ascites.
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Overlay histogram showing HEK293 cells stained with ab57602 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57602, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using a version of the antibody produced in ascites.
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Mitofusin 2 + Mitofusin 1 was immunoprecipitated using 0.5mg Hela whole cell extract, 10ug of Mouse monoclonal to Mitofusin 1 and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10 min under agitation. No antibody was added to the control lane 2 and no extract or antibody was added to contol lane 3. Hela whole cell extract diluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab57602.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Bands: 84kDa:Mitofusin 1; 60kDa bead background: non specific - 48kDa: We are unsure as to the identity of this extra band.This image was generated using a version of the antibody produced in ascites.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (182)
ab57602 は 182 報の論文で使用されています。
- Li D et al. PGC-1α Affects Epileptic Seizures by Regulating Mitochondrial Fusion in Epileptic Rats. Neurochem Res 48:1361-1369 (2023). PubMed: 36454394
- Kwek XY et al. Role of cardiac mitofusins in cardiac conduction following simulated ischemia-reperfusion. Sci Rep 12:21049 (2022). PubMed: 36473917
- Chidipi B et al. The dynamin-related protein 1 is decreased and the mitochondrial network is altered in Friedreich's ataxia cardiomyopathy. Int J Biochem Cell Biol 143:106137 (2022). PubMed: 34923139
- Park HR & Yang EJ Combined Treatment with Herbal Medicine and Drug Ameliorates Inflammation and Metabolic Abnormalities in the Liver of an Amyotrophic Lateral Sclerosis Mouse Model. Antioxidants (Basel) 11:N/A (2022). PubMed: 35052677
- Shin J et al. Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent manner. Redox Biol 50:102252 (2022). PubMed: 35121402