Anti-PDK1 抗体 [2H3AA11] (ab110335)
Key features and details
- Mouse monoclonal [2H3AA11] to PDK1
- Suitable for: ICC/IF, WB, Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PDK1 antibody [2H3AA11]
PDK1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2H3AA11] to PDK1 -
由来種
Mouse -
特異性
This antibody does not cross-react with other isoforms of human PDK (i.e. PDK2, 3 or 4) and does not cross-react with rat or mouse samples. -
アプリケーション
適用あり: ICC/IF, WB, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa cells; Human heart and liver tissue lysates; and HL-60 cells
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特記事項
This monoclonal antibody to PDK1 has been knockout validated in Western blot. The expected band for PDK1 was observed in wild type cells and the band was not seen in knockout cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
2H3AA11 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110335の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
組織特異性
Expressed predominantly in the heart. -
配列類似性
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 5163 Human
- Omim: 602524 Human
- SwissProt: Q15118 Human
- Unigene: 470633 Human
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別名
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
- HGNC:8809 antibody
- Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
see all
画像
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PDK1 knockout HAP1 cell lysate (20 µg)
Lane 3: Human fetal heart lysate (20 µg)
Lane 4: LnCaP cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab110335 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.ab110335 detected the expected band for PDK1 in wild-type cells and the band was not seen in knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDK1 knockout samples were subjected to SDS-PAGE. ab110335 and ab8245 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-PDK1 antibody [2H3AA11] (ab110335) at 1 µg/ml
Lane 1 : Human heart lysate at 10 µg
Lane 2 : Human liver lysate at 10 µg
Lane 3 : Recombinant Human PDK1 at 0.01 µg
Lane 4 : Recombinant Human PDK2 at 0.01 µg
Lane 5 : Recombinant Human PDK3 at 0.01 µg
Lane 6 : Recombinant Human PDK4 at 0.01 µg
Predicted band size: 49 kDa -
All lanes : Anti-PDK1 antibody [2H3AA11] (ab110335) at 1 µg/ml
Lane 1 : HeLa cells incubated for 24 hours with vehicle
Lane 2 : HeLa cells incubated for 24 hours with 100 µM cobalt chloride (CoCl2)
Lane 3 : HeLa cells incubated for 24 hours with 1 mM deferoxamine (DFO)
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
Treatment with either CoCl2 or DFO results in increased expression of PDK1, which is known to be transcriptionally upregulated in response to hypoxia. -
HL-60 cells were stained with 1 µg/mL PDK1 antibody (ab110335) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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Immunocytochemistry image of PDK1 stained HeLa cells. The Upper image shows cells which were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the PDK1 antibody (ab110335) at 2 µg/mL for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
The lower image shows cells co-stained with an antibody against HSP60 (red), an enzyme also located in the mitochondrial matrix. The composite image shows an identical mitochondrial pattern for both antibodies indicated by merged orange color.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab110335 は 6 報の論文で使用されています。
- Sun S et al. MicroRNA let-7i-3p affects osteoblast differentiation in ankylosing spondylitis via targeting PDK1. Cell Cycle 20:1209-1219 (2021). PubMed: 34048311
- Arai MA et al. Evaluation of Naturally Occurring HIF-1 Inhibitors for Pulmonary Arterial Hypertension. Chembiochem 22:2799-2804 (2021). PubMed: 34216084
- Nguyen QL et al. Alterations in platelet bioenergetics in Group 2 PH-HFpEF patients. PLoS One 14:e0220490 (2019). PubMed: 31365585
- Nguyen QL et al. Platelets from pulmonary hypertension patients show increased mitochondrial reserve capacity. JCI Insight 2:e91415 (2017). PubMed: 28289721
- Ahn E et al. Temporal fluxomics reveals oscillations in TCA cycle flux throughout the mammalian cell cycle. Mol Syst Biol 13:953 (2017). PubMed: 29109155
- Goto M et al. Adaptation of leukemia cells to hypoxic condition through switching the energy metabolism or avoiding the oxidative stress. BMC Cancer 14:76 (2014). WB ; Human . PubMed: 24506813