Mitochondrial DNA Isolation Kit (ab65321)
Key features and details
- Assay type: Direct
- Assay time: 3 hr
- Sample type: Adherent cells, Suspension cells, Tissue
製品の概要
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製品名
Mitochondrial DNA Isolation Kit -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Direct -
全工程の試験時間
3h 00m -
製品の概要
Mitochondrial DNA Isolation Kit ab65321 provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications
Please note: One kit is sufficient for 50 samples if Adherent cell or Suspension cells are used and for 25 samples if tissue is used.
Mitochondrial DNA isolation protocol summary:
- collect cells by centrifugation at 600 x g for 5 min and wash with PBS
- centrifuge for 5 min and resuspend pellet in cytosol extraction buffer
- incubate on ice for 10 min
- homogenize in dounce tissue grinder
- centrifuge for 10 min at 700 x g to pellet nuclei and intact cells
- transfer supernatant and centrifuge at 10,000 g for 30 min
- remove supernatant, resuspend pellet in cytosol extraction buffer and centrifuge at 10,000 g for 30 min
- resuspend pellet (mitochondria) and lyse in mitochondrial lysis buffer for 10 min
- add enzyme mix and incubate for 60 min
- add ethanol and incubate for 10 min
- centrifuge for 5 min and retain pellet (mitochondrial DNA) -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K280 Mitochondrial DNA Isolation Kit. K280-50 is the same size as the 1 kit size of ab65321.
Mitochondria are semi-autonomous organelles which functions in aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer's disease, and muscle disorders. Isolation and quantification of mtDNA are often required to study the relationships between the diseases and mtDNA.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 kit 5X Cytosol Extraction Buffer I 1 x 20ml Enzyme B Mix 1 vial Lysis Buffer III 1 x 1.8ml TE Buffer I 1 x 1.5ml -
研究分野
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関連性
Mitochondria are semiautonomous organelles which function in the aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer’s disease, and muscle disorders.
データシートおよび資料
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Datasheet download
参考文献 (56)
ab65321 は 56 報の論文で使用されています。
- Lin WY et al. Probiotics and their Metabolites Reduce Oxidative Stress in Middle-Aged Mice. Curr Microbiol 79:104 (2022). PubMed: 35157139
- Han J et al. Atractylenolide III Improves Mitochondrial Function and Protects Against Ulcerative Colitis by Activating AMPK/SIRT1/PGC-1a. Mediators Inflamm 2022:9129984 (2022). PubMed: 35431653
- Du X et al. Anti-fibrotic mechanism of SPP1 knockdown in atrial fibrosis associates with inhibited mitochondrial DNA damage and TGF-ß/SREBP2/PCSK9 signaling. Cell Death Discov 8:246 (2022). PubMed: 35508610
- Li MZ et al. LPS-Induced Activation of the cGAS-STING Pathway is Regulated by Mitochondrial Dysfunction and Mitochondrial DNA Leakage in Endometritis. J Inflamm Res 15:5707-5720 (2022). PubMed: 36238763
- Zhen Y et al. Flubendazole induces mitochondrial dysfunction and DRP1-mediated mitophagy by targeting EVA1A in breast cancer. Cell Death Dis 13:375 (2022). PubMed: 35440104