Mitochondria Isolation Kit for Tissue (ab110168)
Key features and details
- Assay time: 1 hr
- Sample type: Tissue
製品の概要
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製品名
Mitochondria Isolation Kit for Tissue
Mitochondria Isolation キット 製品一覧 -
サンプルの種類
Tissue -
全工程の試験時間
1h 00m -
製品の概要
ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Mitochondrial isolation protocol summary:
- suspend minced tissue in isolation buffer
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in isolation buffer, spin at 12,000g for 15 min, collect pellet and repeat spin
- resuspend pellet in isolation buffer and analyze or freeze -
特記事項
Tested tissues: brain, liver and heart
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 kit Isolation Buffer 1 x 100ml Wash Buffer 1 x 30ml -
研究分野
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別名
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
関連製品
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Related Products
画像
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Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (58)
ab110168 は 58 報の論文で使用されています。
- Tashiro S et al. Pirfenidone attenuates acetaminophen-induced liver injury via suppressing c-Jun N-terminal kinase phosphorylation. Toxicol Appl Pharmacol 434:115817 (2022). PubMed: 34890640
- Rajab BS et al. Differential remodelling of mitochondrial subpopulations and mitochondrial dysfunction are a feature of early stage diabetes. Sci Rep 12:978 (2022). PubMed: 35046471
- Zhu Y et al. miR-340-5p Mediates Cardiomyocyte Oxidative Stress in Diabetes-Induced Cardiac Dysfunction by Targeting Mcl-1. Oxid Med Cell Longev 2022:3182931 (2022). PubMed: 35126811
- Gomes KP et al. Proteomic Analysis Suggests Altered Mitochondrial Metabolic Profile Associated With Diabetic Cardiomyopathy. Front Cardiovasc Med 9:791700 (2022). PubMed: 35310970
- Czernik M et al. Mitochondrial function and intracellular distribution is severely affected in in vitro cultured mouse embryos. Sci Rep 12:16152 (2022). PubMed: 36167966