Application
Western blot
Sample
Chicken Tissue lysate - whole (E1.5 embryos)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
10 µg
Specification
E1.5 embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.05% Tween-20 + 5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/25000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (H+L) Antibody, Peroxidase-Labeled
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Prime
Exposure
30 second(s)
Bands
Specific: 15 kDa Non-specific: 31, 33, 57, 67, 95, 115 kDa
Positive control
A band with the same size was observed with an anti-FLAG antibody used on a lysate from embryos expressing a midkine construct tagged with a FLAG epitope.
Additional data
Additional Notes
Membrane: nitrocellulose
Lysis buffer: 8M urea, 2.5% SDS
Lane 1: Magic Mark XP Standard
Lane 2: Lysate from E1.5 chicken embryo (Hamburger-Hamilton stage 10)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 23 2017