Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA buffer, pH 7.3
Secondary antibody
Name
Non-Abcam antibody was used: rabbit anti-mouse
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/200
Additional data
Additional Notes
Antigen retrieval was performed in Citric buffer, pH6 using microwave method. Microwave heating was performed for 9 minutes to reach temperature of 98 degrees C, followed by gradual cooling at room temperature
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投稿 May 26 2011