Anti-Methionine Aminopeptidase 2/p67 抗体 (ab123523)
Key features and details
- Rabbit polyclonal to Methionine Aminopeptidase 2/p67
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Methionine Aminopeptidase 2/p67 antibody
Methionine Aminopeptidase 2/p67 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Methionine Aminopeptidase 2/p67 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to Human Methionine Aminopeptidase 2/p67 aa 100-150.
Database link: NP_006829.1 -
ポジティブ・コントロール
- HeLa, 293T, Jurkat, NIH3T3 whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7-8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab123523の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 52 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. -
配列類似性
Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily. -
翻訳後修飾
Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding. -
細胞内局在
Cytoplasm. About 30% of expressed METAP2 associates with polysomes. - Information by UniProt
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参照データベース
- Entrez Gene: 404150 Cow
- Entrez Gene: 10988 Human
- Entrez Gene: 56307 Mouse
- Omim: 601870 Human
- SwissProt: Q3ZC89 Cow
- SwissProt: P50579 Human
- SwissProt: O08663 Mouse
- Unigene: 444986 Human
see all -
別名
- A930035J23Rik antibody
- AI047573 antibody
- AL024412 antibody
see all
画像
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Detection of Methionine Aminopeptidase 2/p67 by Western Blot of Immunprecipitate.
Lane 1: ab123523 at 1µg/ml staining Methionine Aminopeptidase 2/p67 in HeLa whole cell lysate immunoprecipitated using ab123523 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds. -
All lanes : Anti-Methionine Aminopeptidase 2/p67 antibody (ab123523) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab123523 は論文での使用が確認できていません。