Application
Western blot
Sample
Dog Cell lysate - whole cell (osteosarcoma)
Loading amount
30 µg
Specification
osteosarcoma
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA
Secondary antibody
Name
Non-Abcam antibody was used: Competitor Peroxidase labeled anti-rabbit IgG (H+
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
SuperSignal West Dura extended duration
Exposure
1 minute(s) and 0 second(s)
Positive control
Rh30 cells stimulated with rhHGF @ 100ng/mL x15 min
Additional data
Abcam response
Thank you for your feedback. Dog is an untested species and your results suggest that this antibody is not suitable for dog. This will be extremely useful for other researchers. We wish you all the best in your research.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 15 2012