Application: Western Blot
Sample: Mouse whole cell lysate (3T3 SA cell line)
Loading amount: 20ug
Gel Running conditions: reduced denaturating (4-12% gradient gel)
Blocking step: 30 min in 5% milk at room temperature
Dilution: 1/1000
Incubation time: over night at 4°C or 1 hour at room temperature
Secondary antibody: non Abcam goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
Detection: ECL
Sample: Mouse whole cell lysate (3T3 SA cell line)
Loading amount: 20ug
Gel Running conditions: reduced denaturating (4-12% gradient gel)
Blocking step: 30 min in 5% milk at room temperature
Dilution: 1/1000
Incubation time: over night at 4°C or 1 hour at room temperature
Secondary antibody: non Abcam goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
Detection: ECL
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 21 2014