Application
ChIP
Sample
Human Cell lysate - whole cell (HL60(acute promyelotic leuemia cells line))
Specification
HL60(acute promyelotic leuemia cells line)
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: SDS lysis buffer
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: SDS lysis buffer
Detection step
Semiquantitative PCR
Positive control
acetly-histone H3 (cat #06-599, millipore)
Negative control
Rabbit IgG(cat# pp64, millipore)
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: lysis buffer(including inhibitor)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Sungsin Jo
Verified customer
投稿 Oct 23 2009