Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (Mouse 18 DIV cortical neurons)
Specification
Mouse 18 DIV cortical neurons
Treatment
25mM KCl
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Tween 20 + 5% BSA
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 55 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
Mouse 18 DIV cortical neurons were treated with KCl for 0, 10 and 30min and subjected to western blot using MeCP2 (ab2828) antibodies. Gel shift upon KCl treatment was observed indicating KCl-induced phosphorylation.
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投稿 Dec 10 2014