Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - nuclear (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Pierce ECL
Bands
Specific: 72 kDa Non-specific: 70 , 78 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
- N2a
- N2a + MeCP2-e1
- Brain nuclear extract from MeCP2 KO mouse
Positive control
N2a + MeCP2-e2
Additional data
Additional Notes
We have carried out an extensive comparison to our home-made anti-MeCP2 antibodies, both by IF and WB, on transfected cells and mouse brains. The paper describing this work is published in F1000R (refereeing pending ) , and available on http://f1000research.com/articles/2-204/v1
Abcam response
ab2828 is a polyclonal antibody and the immunogen shares homology between the two isoforms so lot to lot variations are very likely. One lot might be specific to -e1, other to -e2 so we should expect different results in WB.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Atnjoly
Verified customer
投稿 Oct 10 2013