Anti-Mannosidase II/MAN2A1 抗体 (ab77353)
Key features and details
- Goat polyclonal to Mannosidase II/MAN2A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Mannosidase II/MAN2A1 antibody
Mannosidase II/MAN2A1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Mannosidase II/MAN2A1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human Placenta lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab77353 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77353の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa).
A 1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyl hydrolase 38 family. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4124 Human
- Omim: 154582 Human
- SwissProt: Q16706 Human
- Unigene: 432822 Human
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別名
- 3-1 antibody
- 6-alpha-mannosidase antibody
- Alpha mannosidase 2 antibody
see all
画像
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Anti-Mannosidase II/MAN2A1 antibody (ab77353) at 0.3 µg/ml + Human Placenta lysate (in RIPA buffer) at 35 µg
Predicted band size: 131 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Mannosidase II/MAN2A1 antibody (ab77353) at 3 µg/ml
Lane 1 : MOCK-transfected HEK293 cell lysate
Lane 2 : HEK293 cell lysate overexpressing Mannosidase II/MAN2A1
Predicted band size: 131 kDa
データシートおよび資料
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Datasheet download
参考文献 (0)
ab77353 は論文での使用が確認できていません。