Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lymph Node, Spleen, Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 4
Permeabilization
No
Specification
Lymph Node, Spleen, Liver
Blocking step
Ultratek HRP (anti-polyvalent), ScyTek Laboratories, Logan, Utah, USA as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Emerald Diluent Antibody, CellMarque.
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Ultratek HRP (anti-polyvalent), ScyTek Labs
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The antibody was tested at 3 dilutions (1:100 1 :200 and 1 :400) and at different antigen retrieval conditions (without antigen retrieval, pH4, pH6 and pH9 for 20 minutes at 90°C). The best conditions were at pH4 and 1:200 dilution. The labelling was specific and strong and the background noise was absent.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sara Belluco
Verified customer
投稿 May 23 2018