Application
Western blot
Sample
Human Cell lysate - whole cell (Human Macrophages)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
Human Macrophages
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye 800CW Goat (polyclonal) anti-Rabbit IgG (H +
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Infrared
Exposure
5 minute(s) and 0 second(s)
Positive control
Human Macrophages are a positive control for the Mannose Receptor
Additional data
Additional Notes
This antibody showed no conclusive results. I would not use this antibody for WB and IHC.
Abcam response
It would appear that using this sample type and protocol, the antibody may not be sensitive enough to detect the mannose receptor. We recommend scientists who are considering the antibody for use in WB to also consider our positive control ab29413 to run along side your samples. This case was resolved in accordance with Abcam's AbPromise Guarantee.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Francesco Elia Marino
Verified customer
投稿 Feb 09 2016