This is a good kit for a rapid protein extraction. However, it is important to know that it is not performing better than a general RIPA buffer or EGFR buffer. Additionally, the protease inhibitor provided with the kit does not contain phosphatase inhibitor. all this info need to be taken in consideration for the selection of a good buffer.
Graph shows comparison between Abcam and RIPA buffer. RIPA buffer performed better in both COV434 and LnCAP with no difference when samples were sonicated using a bath sonicator or probe sonicator.
Graph shows comparison between Abcam and RIPA buffer. RIPA buffer performed better in both COV434 and LnCAP with no difference when samples were sonicated using a bath sonicator or probe sonicator.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Francesco Elia Marino
Verified customer
投稿 Aug 27 2014