Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Dorsal Root Ganglion)
Permeabilization
Yes - 0.2% Triton-X
Specification
Dorsal Root Ganglion
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.2% Triton-X and 3% NDS in 1x PBS
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: Alexafluor 568 Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Additional data
Additional Notes
Antigen retrieval was performed for all antibodies with sodium citrate buffer (10 mM sodium citrate, pH 6.0).
Immunostained with anti-MafA (1:5000 - Red) and anti-Ret (green)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Irene Not_Provided
Verified customer
投稿 Dec 08 2017