Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Sample
Rat Cell (Insulinoma cell line INS-1)
Specification
Insulinoma cell line INS-1
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBS
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 555 goat anti Rabbit invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/600
Additional data
Additional Notes
In the image nuclei was stained with DAPI. Nuclear MafA (red) is present in insulin producing cells (green label localize insulin).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Javier Triñanes
Verified customer
投稿 Jan 19 2015