Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (Human pancreatic Islets and human PANC-1 cell line)
Specification
Human pancreatic Islets and human PANC-1 cell line
Blocking step
ROCHE Western Blocking Reagent as blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Non-Abcam antibody was used: Novex³s Goat Anti-Rab
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/25000
Detection
Detection method
Chemiluminescence, Millipore’s Luminata
Exposure
5 minute(s) and 30 second(s)
Negative control
Lysate of human exocrine tissue and human PANC-1 cell line
Positive control
Lysate of human pancreatic islets and human PANC-1 cell line transfected with synthetic mRNA coding for human MafA
Positive results were achieved with the same samples by ab26405 Anti-MAFA antibody
Additional data
Additional Notes
This antibody is not suitable for detection of human MafA protein by WB. Suitable antibody for detection of MafA protein of human origin is ab26405 Anti-MAFA antibody.
Abcam response
Thank you for submitting this Abreview. As this antibody hasn't been validated for human samples, we are unsure if the antibody is suitable for this species. It may require further optimization.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Tomas Koblas
Verified customer
投稿 Feb 26 2014