Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Human Cell (PANC-1 cell line transfected with mRNA coding for)
Specification
PANC-1 cell line transfected with mRNA coding for
Permeabilization
Yes - 0.05 % Triton X100
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 37°C · Diluent: DAKO antibody diluent
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 555 donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/400
Additional data
Additional Notes
This antibody is not suitable for detection of human MafA protein by ICC/IF. Suitable antibody for detection of MafA protein of human origin is ab26405 Anti-MAFA antibody.
Abcam response
Thank you for submitting this Abreview. As this antibody hasn't been validated for human samples, nor for ICC/IF, we are unsure if the antibody is suitable for this species or application. It may require further optimization. Perhaps a longer incubation time and an antigen retreival step would improve the staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Tomas Koblas
Verified customer
投稿 Feb 26 2014