The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use at an assay dependent concentration.
1/2000. Predicted molecular weight: 10 kDa.
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Belongs to the intercrine beta (chemokine CC) family.
N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
Chemokine (C C motif) ligand 4 like 1, telomeric antibody
Chemokine (C C motif) ligand 4 like 2 antibody
Chemokine CC Motif Ligand 4 antibody
G 26 antibody
G 26 T lymphocyte secreted protein antibody
G-26 T-lymphocyte-secreted protein antibody
Immune activation 2 antibody
LAG 1 antibody
Lymphocyte activation gene 1 antibody
Lymphocyte activation gene 1 protein antibody
Macrophage inflammatory protein 1 beta antibody
Macrophage inflammatory protein 1-beta antibody
Macrophage inflammatory protein 1b2 antibody
MIP1 beta antibody
Monocyte adherence induced protein 5 alpha antibody
PAT 744 antibody
Protein H400 antibody
Secreted protein G 26 antibody
Secreted protein G26 antibody
SIS gamma antibody
Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody
Small inducible cytokine A4 antibody
small inducible cytokine A4-like antibody
Small-inducible cytokine A4 antibody
T cell activation protein 2 antibody
T-cell activation protein 2 antibody
Western blot - Anti-Macrophage Inflammatory Protein 1 beta antibody (ab25129)
All lanes : Anti-Macrophage Inflammatory Protein 1 beta antibody (ab25129) at 1/2000 dilution
Lane 1 : lysate Lane 2 : supernatant
Predicted band size: 10 kDa
ab25129 staining by Western Blot in RAW264.7 cells treated with LPS for 15 hours.
Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 1 beta antibody (ab25129)
ICC/IF image of ab25129 stained Raw264.7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.