Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Loading amount
30 µg
Specification
Skeletal Muscle
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 260, 78, 55, 42, 26 kDa
Additional data
Additional Notes
Edit Abreview 30459
The picture submitted is not the ladder but the actual protein lysate probed with anti-MVP. The ladder used does not react with ECL and was run 2 lanes over (cropped out). I believe the band for MVP is right above the 107 kDa marker. The other bands are non-specific.
The picture submitted is not the ladder but the actual protein lysate probed with anti-MVP. The ladder used does not react with ECL and was run 2 lanes over (cropped out). I believe the band for MVP is right above the 107 kDa marker. The other bands are non-specific.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 30 2012