Anti-IRF4 抗体 [EPR5653] (ab124691)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5653] to IRF4
- Suitable for: WB, IP, IHC-P, ChIP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IRF4 antibody [EPR5653]
IRF4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5653] to IRF4 -
由来種
Rabbit -
特異性
Uniprot ID - Q15306 -
アプリケーション
適用あり: WB, IP, IHC-P, ChIPmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Ramos, HuT 78 and IM 9 cell lysates; IHC-P: Human colon and tonsil tissues. IP: Ramos lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 4.18 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5653 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124691の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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IP |
1/10 - 1/100.
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IHC-P | (3) |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ChIP |
Use 5 µg for 25 µg of chromatin.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ChIP
Use 5 µg for 25 µg of chromatin. |
ターゲット情報
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機能
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes. -
組織特異性
Lymphoid cells. -
関連疾患
Multiple myeloma -
配列類似性
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
翻訳後修飾
Phosphorylation by ROCK2 regulates IL-17 and IL-21 production. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3662 Human
- SwissProt: Q15306 Human
- Unigene: 401013 Human
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別名
- Interferon regulatory factor 4 antibody
- IRF 4 antibody
- IRF-4 antibody
see all
画像
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IRF4 was immunoprecipitated from 0.35 mg Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg with 124691 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg
Lane 2: ab124691 IP in Ramos whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124691 in Ramos whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
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ab124691, at 1/100, staining IRF4 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Chromatin was prepared from SK-MEL-28 cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab124691 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).ChIP was performed according to the literature (PMID: 24267888)
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All lanes : Anti-IRF4 antibody [EPR5653] (ab124691) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : IM 9 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 52 kDa -
ab124691, at 1/100, staining IRF4 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of dog spleen tissue, staining IRF4 with ab124691.
Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a TRIS-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/50 in PBS plus 1X casein) for 1 hour 30 minutes at 37°C. An undiluted HRP-conjugated horse anti-rabbit polyclonal IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab124691 は 2 報の論文で使用されています。
- Sun R et al. Prognostic significance of interferon regulating factor 4 in esophageal squamous cell carcinoma. Biochem Biophys Res Commun 506:685-691 (2018). PubMed: 30376993
- Vieyra-Garcia PA et al. STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides. Clin Cancer Res 22:3328-39 (2016). IHC-P ; Human . PubMed: 26851186