Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Asthma lung)
Specification
Asthma lung
Fixative
Formaldehyde
Permeabilization
No
Other product details
Concentration
4 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-BSA 1%
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
MUC5B is visualized in green and nucleus in blue (DAPI)
Supplementary method:
- Ab77995 was coupled to biotin using ChromaLink Biotin Protein Labelling Kit (Solulink).
Final concentration of the antibody (biotinylated) is 383µg/ml.
- Streptavidin 488 (1/250) was used to amplifying the signal
Supplementary method:
- Ab77995 was coupled to biotin using ChromaLink Biotin Protein Labelling Kit (Solulink).
Final concentration of the antibody (biotinylated) is 383µg/ml.
- Streptavidin 488 (1/250) was used to amplifying the signal
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Francois Daubeuf
Verified customer
投稿 Apr 27 2011