Anti-MUC5B 抗体 [19.4E] (ab77995)
Key features and details
- Mouse monoclonal [19.4E] to MUC5B
- Suitable for: ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Human, Monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-MUC5B antibody [19.4E]
MUC5B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [19.4E] to MUC5B -
由来種
Mouse -
特異性
Does not react with human Mucin 5A or 5C.
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アプリケーション
適用あり: ELISA, IHC-P, IHC-Fr, WBmore details -
種交差性
交差種: Mouse, Human, Monkey -
免疫原
Synthetic peptide corresponding to Human MUC5B (N terminal). Un-glycosylated, serine and threonine free synthetic peptide from the N terminal region of human MUC5B.
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特記事項
This product was changed from ascites to tissue culture supernatant on 31st May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
19.4E -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77995の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
Deglycosylating the tissue is critical to expose epitope. |
IHC-Fr | (1) |
Use at an assay dependent concentration.
|
WB |
Use at an assay dependent concentration.
|
特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Deglycosylating the tissue is critical to expose epitope. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. -
組織特異性
Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas. -
配列類似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 HAT repeats.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
ドメイン
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
翻訳後修飾
Highly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 727897 Human
- Entrez Gene: 74180 Mouse
- Omim: 600770 Human
- SwissProt: Q9HC84 Human
- SwissProt: Q8K1G6 Mouse
- Unigene: 523395 Human
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別名
- 2300002I04Rik antibody
- A130042M24 antibody
- AV085033 antibody
see all
画像
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ab77995 staining MUC5B in murine asthma lung tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with formaldehyde, followed by incubation with the primary antibody at 4µg/ml for 16 hours at 4°C. ab77995 was coupled to biotin and Streptavidin 488 (1/250) was used to amplify the signal. Counterstained with DAPI (blue).
This image was generated using the ascites version of the product.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab77995 は 23 報の論文で使用されています。
- Wang Z et al. SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages. Cell Mol Immunol 19:210-221 (2022). PubMed: 34983944
- Huang X et al. MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model. Respir Res 23:11 (2022). PubMed: 35042537
- Gurumurthy RK et al. Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection. Nat Protoc 17:1658-1690 (2022). PubMed: 35546639
- Ciccimarra R et al. The normal and fibrotic mouse lung classified by spatial proteomic analysis. Sci Rep 12:8742 (2022). PubMed: 35610327
- Devadoss D et al. Immunomodulatory LncRNA on antisense strand of ICAM-1 augments SARS-CoV-2 infection-associated airway mucoinflammatory phenotype. iScience 25:104685 (2022). PubMed: 35789750