Application
Western blot
Sample
Human Cell lysate - other (HeLa WT vs HeLa rho-0)
Loading amount
13 µg
Specification
HeLa WT vs HeLa rho-0
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Santa Cruz sc-2054 mouse/human adsorbed
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
Pierce West Dura
Exposure
10 minute(s) and 0 second(s)
Positive control
Mitochondrial lysate of HeLa WT cells
Negative control
Mitochondrial lysate of HeLa rho-0 cells. These cells do not have mitochondrial DNA
Additional data
Additional Notes
PVDF membrane (Pierce), lysis buffer 10 mM Tris, pH=8.0, 0.5% SDS, 1x protease inhibitor cocktail Pierce PI-87875. Protein determination: BCA (Pierce)
Abcam response
Thank you for your honest and valuable feedback. We are sorry this antibody has not worked in your hands. Many thanks for your feedback on this antibody, we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 27 2012