Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Zebrafish Cell (Whole Body)
Specification
Whole Body
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T 5% BSA
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) Antibod
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Abcam response
Many thanks for your valuable feedback. It appears that under these experimental conditions, ab110258 does not specifically detect MTCO2 in Zebrafish. We welcome further customer feedback. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 25 2014